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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of rLOX-PP on FAK (Tyr397) and ERK (Thr202/Tyr204) phosphorylation induced by VEGF in HUVECs: (a) HUVECs were treated with rLOX-PP and vehicle ctrl. After treatment, cells were treated with 10 ng/mL of VEGF for 15 min. Whole cell extract were prepared and the samples were subjected to western blotting using antibodies against pFAK (Tyr397), total FAK, pERK (Thr202/Tyr204), total ERK and β–actin was used as loading control. The full-length blots are represented in Supplementary Figs 12, 13, 14, 15 and 16. (b) Bar graph represents the quantification of western blot using ImageJ software for pFAK (Tyr397) and (c) pERK (Thr202/Tyr204). (d) HUVECs were treated with rLOX-PP without stimulation of VEGF. Cell lysate were subjected to western blotting using pFAK (Tyr397), total FAK, pERK (Thr202/Tyr204), total ERK and β–actin. The full-length blots are represented in Supplementary Figs 12, 13, 14, 15 and 16. (e) Densitogram of the western blot shows the ratio of pFAK to total FAK, normalized with β-actin. (f) Densitogram of the western blot shows the ratio of pERK to total ERK, normalized with β-actin. Values were expressed as mean ± SD, n = 3. **p < 0.01, *p < 0.05 versus VEGF + Vehicle ctrl. ^#p < 0.05 versus CTRL. (CTRL – Control; Vehicle ctrl – Vehicle control).