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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uptake and localization of rLOX-PP in HUVECs. (a) LOX-PP protein expression was increased upon addition of rLOX-PP in HUVECs when compared with vehicle ctrl. The full-length blots are represented in Supplementary Figs 17 and 18. (b) Densitogram of the western blot shows LOX-PP protein normalized with β-actin. (c) Western blot for His-tag indicates rLOX-PP with His-tag which was added exogenously to HUVECs was found in the cell lysate indicating its uptake. The full-length blots are represented in Supplementary Figs 19 and 20. (d) Densitogram of the western blot shows His-tagged LOX-PP protein expression normalized with β-actin. Values were expressed as mean ± SD, n = 3. **p < 0.01 versus vehicle ctrl. (Vehicle ctrl – Vehicle control). (e) Increased LOX-PP expression is observed upon addition of rLOX-PP to HUVECs under LSM 880 confocal laser scanning microscope. Cells were stained with Alexa 488 and counterstained with DAPI.