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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient transfection of LOX-PP, confirmation by western blot and mass spectrometry: (a) mRNA expression of LOX-PP overexpressed in HUVECs after 48 h (b) After 48 h of transfection, cell extracts were collected and probed using LOX-PP antibody by western blot and was normalized with β- actin (EV- empty vector, LOX-PP-LOX-PP transfected cells). The corresponding full-length blots are represented in Supplementary Figs 3 and 4. (c) Conditioned medium was collected and it was immunoprecipitated using LOX-PP antibody, immunoprecipitated samples were subjected to western blot using antibody against LOX-PP and coomassie staining was considered as loading control, the corresponding full-length blots are represented in Supplementary Figs 5 and 6. (d) Mass spectrum of LOX-PP in cell lysate. (e) Mass spectrum of LOX-PP in conditioned medium. (f) Immunofluorescence for LOX-PP in HUVECs after overexpression (Magnification 63x).Cells were stained with Alexa 488 and counterstained with DAPI.