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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of LOX-PP on VEGF induced cell migration in HUVECs: (a) For scratch assay HUVEC cells were induced with 10 ng/mL of VEGF, 10 ng/mL of VEGF + EV and 10 ng/mL of VEGF + LOX-PP for 18 h and were imaged using Axio observer microscope at 5x magnification. (b) Bar graph represents the quantification of scratch assay. (c) For transwell migration assay HUVEC cells were induced with 10 ng/mL of VEGF, 10 ng/mL of VEGF + EV and 10 ng/mL of VEGF + LOX-PP for 8 h and were imaged using Axio observer microscope at 5x magnification. (d) Bar graph represents the quantification of transwell migration assay. Cells were counted using ImageJ software (expressed as % of controls). (e) HUVEC cells were induced with 10 ng/mL VEGF, 10 ng/mL VEGF + Vehicle ctrl and 10 ng/ml of VEGF + 2.5 μg/ml of rLOX-PP for 18 h and were imaged using Axio observer microscope at 5 x magnification. (f) Bar graph represents the quantification of scratch assay. Values were expressed as mean ± SD, n = 3. ***p < 0.001 versus VEGF + EV. ***p < 0.001 versus VEGF + Vehicle ctrl. #p < 0.05 versus CTRL. (CTRL – Control; EV – Empty vector; Vehicle ctrl – Vehicle control).