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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of LOX-PP on VEGF induced cell attachment and reorganization of F-actin: (a) Bar graph represents the quantification of cell attachment after overexpression of LOX-PP, attached cells were counted using ImageJ software (expressed as % of controls). (b) Bar graph represents the quantification of cell attachment after addition of rLOX-PP, attached cells were counted using ImageJ software (expressed as % of controls). (c) Inhibitory effect of overexpressed LOX-PP on the reorganization of actin cytoskeleton induced by VEGF (10 ng/mL) under LSM 880 confocal laser scanning microscope. HUVECs were fixed and stained for filamentous actin with phalloidin FITC conjugate. Cells were also transfected with EV. Nucleus is counter-stained with DAPI. Values were expressed as mean ± SD, n = 3. **p < 0.01 versus VEGF + EV. **p < 0.01 versus VEGF + Vehicle ctrl. ^#p < 0.05 versus CTRL. (CTRL – Control; EV – Empty vector; Vehicle ctrl – Vehicle control).