Table 2.
Combination indexes (CI) were calculated according to the Chou-Talay method from the data obtained from fluorescence vs. inhibitor(s) dose from the mCherry-PRprec-EGFP-based assay measured by flow cytometry and from the drug susceptibility assay with NL4-3 virus and the predominating effect (additive for CI = 1, synergism for CI < 1 and antagonism for CI > 1) is shown in bold.
| Drug combination indexes CI in two independent assays | CI:EC50 | CI:EC75 | CI:EC90 | CI:EC95 | Result |
|---|---|---|---|---|---|
| DRV + ATV Virus | 0.72 | 0.55 | 0.42 | 0.36 | Synergism |
| DRV + ATV Total PR inhibition | 1.05 | 0.93 | 0.84 | 0.79 | Additive/Synergism |
| DRV + ATV Precursor inhibition | 1.03 | 1.05 | 1.07 | 1.08 | Additive |
| ATV + NFV Virus | 0.63 | 1.19 | 2.38 | 3.93 | Antagonism |
| ATV + NFV Total PR inhibition | 1.27 | 4.20 | 15.9 | 41.9 | Antagonism |
| ATV + NFV Precursor inhibition | 1.43 | 1.31 | 1.20 | 1.13 | Antagonism |