Fig. 3.

Effects of ECN on the activation of ARE/Nrf2 signaling. (A) PC12 cells were treated with 10 μM ECN for the indicated times. Nrf2 in the cytoplasm and nucleus and HO-1 in the cytoplasm were determined by Western blotting. (B and C) Cells were treated for the indicated times with 10 μM ECN (B) or 1 h with the indicated concentrations of ECN (C), and ARE-luciferase activity was measured. (D) Cells were transfected with 50 nM control siRNA (si Con) or Nrf2-targeted siRNA (si Nrf2) for 48 h and then treated with 10 μM ECN for 6 h.