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. 2018 Jun 1;18:6–15. doi: 10.1016/j.redox.2018.05.015

Fig. 5.

Fig. 5

Nrf2/HO-1 signaling-mediated neuroprotective effects of ECN. (A) PC12 cells were pretreated with SnPP (10 or 20 μM) for 1 h before treatment with ECN (2.5, 5, and 10 μM) for 24 h and then exposed to 250 μM 6-OHDA for an additional 24 h. Cell viability was determined by the MTT assay. Data are presented as the mean ± SD. ###P < 0.001 indicates a significant difference from the 6-OHDA untreated control group; *P < 0.05, **P < 0.01, and ***P < 0.001 indicate a significant difference compared with the 6-OHDA-only exposed group; &&P < 0.01 and &&&P < 0.001 versus the 10 μM ECN plus 6-OHDA treated group. (B) Cells were transfected with 50 nM control siRNA (si Con) or Nrf2-targeted siRNA (si Nrf2) for 48 h and then treated with 10 μM ECN. After 24 h, cells were exposed to 250 μM 6-OHDA for an additional 24 h.