Figure 1.

KL1333 increases the intracellular NAD⁺/NADH ratio. (A) Reaction mixtures consisting of rhNQO1, NAD⁺, NADH, and KL1333 were incubated at 37°C for 1 h, and then NAD⁺ and NADH levels were measured using a microplate reader. (B) C2C12 myoblasts were treated with 1 μM KL1333 for 30 min. Intracellular NAD⁺ and NADH were extracted, and NAD⁺ and NADH levels were measured using a microplate reader. NAD⁺/NADH ratio was calculated based on the concentration of NAD⁺ and NADH. (C) L6 myoblasts were treated with 2 μM KL1333 for 30 min. Intracellular NAD⁺ and NADH were extracted, and then NAD⁺ and NADH levels were measured using a microplate reader. NAD⁺/NADH ratio was calculated based on the concentration of NAD⁺ and NADH. Each experiment was repeated four times. Error bars indicate ±SEM. ^*P < 0.05.