Skip to main content
. 2018 Jul 5;9:552. doi: 10.3389/fneur.2018.00552

Figure 2.

Figure 2

KL1333 activates SIRT1 and AMPK. (A) C2C12 and L6 myoblasts were treated with 1 and 2 μM KL1333 for 1 h, respectively. SIRT1 activities in both cell lines were analyzed using a fluorescence-based assay. (B) C2C12 myoblasts were treated with 1 μM KL1333 for the indicated times. The activity of AMPK was examined by Western blotting using the indicated antibodies. (C) Quantification of pAMPK levels in C2C12 cell lysates using its band density normalized to that of total AMPK. (D) Quantification of pACC levels in C2C12 cell lysates using its band density normalized to that of total ACC. (E) L6 myoblasts were treated with 2 μM KL1333 for the indicated times. The activity of AMPK was examined by Western blotting using the indicated antibodies. (F) Quantification of pAMPK levels in L6 cell lysates using its band density normalized to that of total AMPK. (G) Quantification of pACC levels in L6 cell lysates using its band density normalized to that of total ACC. Each experiment was repeated three times. Error bars indicate ± SEM. *P < 0.05.