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. 2018 Jul 5;9:552. doi: 10.3389/fneur.2018.00552

Figure 3.

Figure 3

KL1333 activates AMPK in a NQO1-dependent manner. (A) L6 myoblasts were pretreated with 250 nM ES936 or 10 μM quercetin for 1 h, and then treated with 2 μM KL1333 for the indicated times. The activity of AMPK was examined by Western blotting using the indicated antibodies. (B) Quantification of the pAMPK/AMPK ratio in ES936 and KL1333-treated cells. (C) Quantification of the pACC/ACC ratio in ES936 and KL1333-treated cells. (D) Quantification of the pAMPK/AMPK ratio in quercetin and KL1333-treated cells. (E) Quantification of the pACC/ACC ratio in quercetin and KL1333-treated cells. Each experiment was repeated three times. Error bars indicate ± SEM. *P < 0.05: significance with respect to the control group. #P < 0.05: significance with respect to the ES936- or quercetin-treated group.