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. 2018 Jul 5;9:552. doi: 10.3389/fneur.2018.00552

Figure 4.

Figure 4

KL1333 induces PGC-1α activation. (A) C2C12 myoblasts were transfected with FLAG-tagged mPGC-1α for 48 h, and then treated with 2 μM KL1333 for the indicated times. Cell lysates were subjected to immunoprecipitation using antibody against FLAG. The acetylation levels of PGC-1α were examined by Western blotting using anti-acetyl lysine antibody. Histograms show the levels of acetylation of PGC-1α relative to total FLAG-tagged mPGC-1α under each of the indicated conditions. The experiment was repeated three times. (B) C2C12 myoblasts were transfected with FLAG-tagged mPGC-1α for 48 h, and then treated with 2 μM KL1333 for the indicated times. Cell lysates were subjected to immunoprecipitation using antibody against phospho-serine. The phosphorylation levels of PGC-1α were examined by Western blotting using an anti-FLAG antibody. Histograms show the levels of phosphorylation of PGC-1α relative to total FLAG-tagged mPGC-1α under each of the indicated conditions. The experiment was repeated three times. (C) Cells expressing PGC-1α luciferase were treated with 1 μM KL1333 for 24 h. Cell lysates were subjected to luciferase assays, and the activity of the PGC-1α promoter was analyzed. The experiment was repeated four times. Error bars indicate ± SEM. *P < 0.05.