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. 2018 Jul 5;9:552. doi: 10.3389/fneur.2018.00552

Figure 7.

Figure 7

KL1333 improves mitochondrial mass and OXPHOS in MELAS fibroblasts. MELAS 1 fibroblasts were treated with 1 μM KL1333 for 24 h. (A) Cells were stained with MitoTracker Green FM, and mitochondrial mass was measured using a flow cytometer. (B) Cells were stained with TMRM, and mitochondrial membrane potential was measured using a flow cytometer. (C) Protein levels of mitochondrial complex subunits were analyzed by Western blotting using the indicated antibodies. (D) Quantification of mitochondrial complex subunit proteins using their band densities normalized to that of VDAC. (E) MELAS 1 fibroblasts were treated with 1 μM KL1333 for 24 h, and OCR was measured using the Seahorse XF24 analyzer. Oligomycin, FCCP, and antimycin A were sequentially added at the indicated time points (arrow). (F) Basal OCR and AUC OCR are shown in the histogram. Each experiment was repeated three times. Error bars indicate ± SEM. *P < 0.05.