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. 2018 Jul 5;11:230. doi: 10.3389/fnmol.2018.00230

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Copyright © 2018 Wickert, Hildick, Baillie, Jelinek, Aparisi Rey, Monory, Schneider, Ross, Henley and Lutz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface expression of HA-CB1F238L compared with HA-CB1wt. (A) HEK293 cells stably expressing HA-CB1wt or HA-CB1F238L were treated with trypsin or versene (control). Trypsin is able to cleave the extracellular HA-tag. Thus, control samples show total CB1 receptor amount, whereas trypsin treated samples show intracellular CB1 receptor amount. (B) Surface HA-CB1 was calculated and expressed as percent of total HA-CB1. We found 85.49% (±1.44) of HA-CB1wt, but only 57.68% (±4.26) of HA-CB1F238L to be located to the plasma membrane. (Student’s t-test. Data are presented as the mean ± SEM of n = 7 independent experiments. ***p < 0.001).