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. 2018 Jul 5;9:1538. doi: 10.3389/fimmu.2018.01538

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Copyright © 2018 Boulanger, Eccleston, Phillips, Coveney, Elliott and Dalchau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell surface abundance can be predicted by a peptide competition metric. The peptide competition metric F_ratio (Eq. 3) was calculated using measurements of cytoplasmic peptide abundance for SSLENFRAYV and variants of ASNENMETM (fluorescence units in Figures 1 and 3). F_ratio (horizontal axis) was then compared with experimental measurements of cell surface abundance of SSLENFRAYV (vertical axis). Different colored traces represent different cytoplasmic levels of the competing ASNENMETM variant, as indicated in Figure 1B. A to F represent competitions between SSLENFRAYV and ASNENLETM (A), ASIENMETM (B), ASNENMETV (C), ASNENMETI (D), ASNENMETM (E) and ASIENLETM (F).