Figure 8.

Confocal microscopy analysis of CXCL10 expression in tuberculosis (TB) [lines, (A–C)] and control [line, (D)] samples. Lung sections were stained with anti-CD14 (a, e, i, m, green), with anti- CXCL10 (b, f, j, n, red), and with anti-CD4 (e, g, k, o, blue). In TB samples, the merged images of the triple-fluorescence signals (d, h, l, merge) highlights the expression of CXCL10 in CD4⁺ T lymphocytes (purple) presents in the inflammatory area and in macrophages (yellow); some CD14⁺ positive cells do not express CXCL10 (green). In controls, merged image (p) shows the CXCL10 expression only in CD14⁺ positive cells (yellow). Scale bars: (a, d) (m−p) = 25 µm; (e−h) = 8 µm.