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. 2018 Feb 6;16(8):1424–1433. doi: 10.1111/pbi.12884

Figure 1.

Figure 1

Schematic diagram of the Cas9/sgRNA binary vector and target site selection in the AcPDS gene. (a) Schematic illustrating the four sgRNAs targeting the AcPDS gene. Orange boxes indicate exons, black lines represent introns and F1/R1 and F2/R2 indicate binding sites of the primers used for PCR amplification. (b) Schematic depicting the paired‐sgRNA expression cassettes of both the CRISPR/Cas9 and the PTG/Cas9 systems. Diamonds with different colours represent sgRNA, rectangles represent the sgRNA scaffold and purple round rectangles represent tRNA. (c) The architecture of paired‐sgRNA/Cas9 binary vectors for both the CRISPR/Cas9 and the PTG/Cas9 systems. (d) The sequences of the four sgRNAs. Red letters indicate the PAM motif of each sgRNA.