Skip to main content
. 2018 Feb 6;16(8):1424–1433. doi: 10.1111/pbi.12884

Figure 4.

Figure 4

Chromosomal fragment deletion induced by paired‐sgRNA/Cas9 in kiwifruit. (a) PCR detection of chromosomal fragment deletion between sgRNA1 and sgRNA2. The truncated PCR products after agarose gel electrophoresis are shown. WT, wild type. The left gel image shows the biallelic mutant and the right is chimeric mutant. (b) PCR detection of chromosomal fragment deletion between sgRNA3 and sgRNA4. The truncated PCR products after agarose gel electrophoresis are presented. (c) to (d) Alignment of representative sequences of chromosomal fragment deletion and wild‐type sequence. The sgRNA region is labelled in red colour, and the PAM region is shown in green letters. The sequencing chromatograms of the corresponding regions are shown below the alignment. The sequencing chromatograms at the sgRNA2 region were the results sequenced from the reverse direction.