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Off‐target analysis of kiwifruit callus lines
| Target | Off‐target sites | Putative off‐target sequences | Putative off‐target loci | Number of examined lines | Number of lines with off‐targets |
|---|---|---|---|---|---|
| gRNA1 | 1 | tAGGTCTtTCCCATCAAGAT | Chr23: ‐318123 | 78 | 0 |
| 2 | ACTCGATGGAcGAaGATATA | Chr23: ‐307000 | 78 | 0 | |
| gRNA3 | 3 | ATGGgTTAACTGTTAAAGAC | Chr23: ‐306730 | 78 | 0 |
| gRNA4 | 4 | ATGGTTTAACTGTTtgAGAC | Chr28: ‐392519 | 78 | 0 |
