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. 2018 Mar 8;16(8):1488–1501. doi: 10.1111/pbi.12891

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© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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VpPR10.1 interacts with VpVDAC3. (a)Yeast two‐hybrid assay. pGBKT7 or pGADT7 plasmid containing VpPR10.1 and VpVDAC3 was transformed into Y2H Gold. Combinations of the (AD/T) with BD/p53 and BD/Lam were used as positive and negative controls. (b) Bimolecular fluorescence complementation (BiFC) assay in vivo. Merged fluorescent and visible light images were taken. Bars = 10 μm. Auto, chloroplast auto‐fluorescence. (c) Pull‐down assay. The presence or absence of each protein in the final mixture is indicated as + or −, respectively. (d) Co‐IP and immunoblotting (IB) of GFP/Flag‐VpVDAC3 and VpPR10.1‐GFP/Flag‐VpVDAC3 were co‐expressed in Nicotiana benthamiana leaves. Flag antibodies were used for the detection of immunoprecipitated VpPR10.1‐GFP and Flag‐VpVDAC3.