FIGURE 1.

Identification of Ssb_Xoc transgenic N. benthamiana plants. (A) Construction of vector expressing Ssb_Xoc in transgenic N. benthamiana. The CaMV promoter and nopaline synthase (NOS) polyadenylation signal are shown in black solid rectangles and flanked the Ssb_Xoc coding region. (B) PCR analysis of transgenic lines using the Ssb_Xoc-specific primers. Lane M, molecular weight marker; lanes 1-22 represent different transgenic lines, and lanes 2 and 21 are lines OE1 and OE9, respectively. Lane 23 contains the positive control, and the arrow shows the location of the 552-bp Ssb_Xoc PCR product. (C) Southern blot hybridization of transgenic lines, OE1 and OE9, with dig-labeled Ssb_Xoc. Lanes: M, molecular weight marker; CK, check, pCAMBRIA2300-Ssb_Xoc; WT, wild-type N. benthamiana; OE1 and OE9, transgenic lines containing Ssb_Xoc gene. (D) Expression measurement of Ssb_Xoc gene in transgenic lines OE1 and OE9 by RT-PCR. The housekeeping gene, EF1α, was used as an internal control for normalizing the data.