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. 2018 Jul 5;9:953. doi: 10.3389/fpls.2018.00953

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Copyright © 2018 Cao, Yang, Ma, Sun and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The oxidative burst assay in WT and Ssb_Xo_c transgenic N. benthamiana plants inoculated with Hpa1 and Pst DC3000. WT and transgenic plants were injected with Hpa1 protein (10 μg ml^-1) and Pst DC3000 pathogen (OD = 0.01), and at 6 hpi, treated leaves were collected. (A) Visualization of H₂O₂ accumulation by DAB staining in leaves inoculated with Hpa1 and Pst DC3000. (B) Evaluation of H₂O₂ levels in leaves. Upper panel shows H₂O₂ levels in WT and transgenic lines inoculated with empty vector preparation (EVP; negative control) and Hpa1; Lower panel shows H₂O₂ levels in WT and transgenic lines inoculated with 10 mM MgCl₂ (negative control) and Pst DC3000. (C) Phenotypes of WT and transgenic lines inoculated with Hpa1 and Pst DC3000 after 24 h. Inoculation sites are indicated with open circles. Error bars represent SD, and values with different letters are significant at P < 0.05.