Table 1.
Genes | Primer sequences (5′ – 3′) |
Purpose | |
---|---|---|---|
Forward | Reverse | ||
SsbXoc | CGGGATCCATGGCCCGCGGCATCAATAAAGT | CCTCTAGATCAGAACGGGATATCGTCGTCGGC | Cloning |
SsbXoc | ATGGCCCGCGGCATCAATAAAGT | TCAGAACGGGATATCGTCGTCGGC | RT-PCR, Probe |
EF1α | AGACCACCAAGTACTACTGCAC | CCACCAATCTTGTACACATCC | RT-PCR |
SsbXoc | CAGGGTGATGGTGGATACGG | ATATCGTCGTCGGCGAAATC | qRT-PCR |
PR1a | GGTGTAGAACCTTTGACCTGGG | AAATCGCCACTTCCCTCAGC | qRT-PCR |
PR2 | TAGAGAATACCTACCCGCCC | GAGTGGAAGGTTATGTCGTGC | qRT-PCR |
PR4 | GTGACGAACACAAGAACAGGAA | CCACTCCATTTGTGTCCAAT | qRT-PCR |
SGT1 | CCTTCTATGAGCAGACATCCCA | GCGTCCAGTATGACAACCCA | qRT-PCR |
HIN1 | TGCGTCCAGTATTCAAAGGTCA | GCTTCACTTCCATCTCATAAACCC | qRT-PCR |
HSR203J | TGCGTCCAGTATTCAAAGGTCA | GCTTCACTTCCATCTCATAAACCC | qRT-PCR |
MPK3 | CGGCACATGGAACACG | GACCGAATAATCTGATGAAGG | qRT-PCR |
APX | TGGAACCCATCAAGGAGCAG | ATCAGGTCCTCCAGTGACTTC | qRT-PCR |
GPX | GTTTCCGCTAAGAGATTTGAGTTG | CCCTTAGCATCCTTGACAGTG | qRT-PCR |
CAT1 | AACAAGGCTGGGAAATCAACC | TGGCTGTGATTTGCTCCTCC | qRT-PCR |
EXPA1 | TTGTTTCTCTGCTTCTGGATGG | CTTAATGCAGCAGTGTTTGTACCA | qRT-PCR |
EIN2 | GGCATAATAGATCTGGCATTTTCC | TATCTAAGAGCATCGGTGCAGTTG | qRT-PCR |
EF1α | AGACCACCAAGTACTACTGCAC | CCACCAATCTTGTACACATCC | qRT-PCR |