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Pathway for redox balanced ethanol production in C. bescii. The AdhE from C. thermocellum requires two NADH to generate ethanol from acetyl-CoA, but glycolysis in C. bescii generates one NADH and one reduced ferredoxin (Fdred). The heterologous expression of Rnf allows for the transfer of electrons from reduced ferredoxin to NAD to generate the additional NADH needed for redox balanced ethanol production.
