Figure 6.

Longitudinal expression of SASP senescence gene Cxcl5 over the course of treatment and relapse. C57Bl6/j mice were orthotopically transplanted with two different Trp53^+/+, MMTV-Wnt1 tumors that express luciferase under the control of the Cxcl5 promoter region. Tumor volume was determined (solid lines, solid squares), and mice were imaged for luciferase expression (dashed line, open circles) regularly. Mice were left untreated (A) or were treated with five consecutive IP injections of 4 mg/kg doxorubicin (B) as indicated by arrowheads on the X-axis. Data are expressed graphically with tumor volume indicated by solid lines on the left Y-axis and luciferase activity normalized to tumor volume indicated by dashed lines on the right Y-axis.