Figure 1.

H₂S downregulates the expression of MGAT5 and inhibits its activity.

(A). After treatment with various concentrations of NaHS for 24 h, cell extracts were prepared and applied to immunoblotting with MGAT5. GAPDH was used as a loading control. (B). quantitative real-time PCR analysis the expression of MGAT5 mRNA in GC cells after 24 hours with treatment NaHS at various concentrations. (C). Immunofluorescence staining of MGAT5 in GC cells treated with NaHS at 100 μM after 24 hours. (D). The effect of H₂S on MGAT5 activity inhibition in different cells. Various concentrations of NaHS were added to GC cells. The activity of MGAT5 was determined by the HPLC methods using pyridiylaminated GlcNAc₂Man₃GlcNAc₂ as acceptor substrate in the absence of Mn²⁺. Each bar represents the means ± S.D. of three independent experiments.