Figure 3.

H₂S suppresses MGAT5-promoted GC cells growth.

(A). FACS analysis of apoptosis in GC cells after 24 hours with treatment NaHS at various concentrations. (B). After treatment with various concentrations of NaHS for 24 h, cell extracts were prepared and applied to immunoblotting with apoptosis relevant protein. GAPDH was used as a loading control. (C). BrdU proliferation assay measuring GC cells proliferation capacity with NaHS treatment on various concentrations. Seahorse XF24 Extracellular Flux Analyzer examined the inhibitory effect of H₂S on cellular metabolism capacity of serum free stimulated stably MGAT5 over-expression in GC cells with NaHS treatment for 200 minutes at 100 μM. (D). Glycolytic Function. (E). Mitochondrial Respiration. (F). Fatty Acid Oxidation. (G). The effect of NaHS solution on 2-deoxtglucose uptake in GC cells. (H). Chemiluminescence analysis assayed the level of ROS in GC cells after 24 hours with treatment NaHS at various concentrations. Each bar represents the means ± S.D. of three independent experiments.