H2S inhibits MGAT5-promoted GC cells migration and invasion.
(A). Wound healing assayed that the inhibitory effect of H2S to migration for serum free stimulated GC cells. Up panel: treatment of H2S, the migration capacity was detected. a. BGC823, b. MGC803, and c. MKN45. Down panel: quantification of the inhibition activity of H2S on migration. (B). Transwell assayed that the inhibitory effect of H2S to invasion for serum free stimulated GC cells. Up panel: treatment of H2S, the invasion capacity was detected. a. BGC823, b. MGC803, and c. MKN45. Down panel: quantification of the inhibition activity of H2S on invasion. (C). Western blotting assayed EMT relevant protein on GC cells after 24 hours of treatment with NaHS at various concentrations. GAPDH was used as a loading control. (D). The inhibitory effect of H2S on migration of serum free stimulated MGAT5 over-expression in BGC823. Each bar represents the means ± S.D. of three independent experiments.