Figure 5.
H2S inhibits tumor growth and metastasis in gastric carcinoma Xenografts.
(A). Effect of H2S on lung metastasis of human gastric carcinoma cell BGC823 in mice orthotopic Xenotransplantation model. Left. representative bioluminescence imaging of metastatic nodules on lungs. Right. The BGC823 colonies were measured. (n = 3 flanks and 4 mice in each group). (B). Effect of H2S on lung metastasis of human gastric carcinoma cell BGC823 in mice orthotopic Xenotransplantation model. Left. representative bioluminescence imaging of metastatic nodules on lungs. Right. HE staining and IHC staining of Ki67 representative photograph of metastatic nodules on lungs. (C). Tumor growth inhibition upon H2S treatment in BGC823 gastric carcinoma mice subcutaneous Xenografts tumor model. a. The curve of tumor growth after 15-days treatment of H2S. b. Experimental inhibitory effects of H2S on BGC823 Xenografts in nude mice. The percentage of relative tumor volume inhibition values was measured on the last day during the experiment. (D). Effect of H2S against primary tumor growth and angiogenesis. A typical photograph of IHC staining of MGAT5, CD31, and cleaved-caspase-3. (E). Inhibition of the expression of MGAT5 in BGC823 mice orthotopic Xenotransplantation model by H2S. Mice were humanely euthanized on the last day at 2 hours post-administration of H2S and the tumors were resected. Equal amounts of proteins of tumor tissues were evaluated for expression of MGAT5 levels. (F). The level of H2S in the plasma of mice orthotopic Xenotransplantation model. Data are shown as means ± S.D. (n = 3 flanks and 4 mice in each group).