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. 2018 Jul 5;9:871. doi: 10.3389/fphys.2018.00871

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Copyright © 2018 Solari, Marcozzi, Negrini and Moriondo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of osmolarity on the end diastolic diameter (A), contraction amplitude (B) and stroke volume (SV, C) in spontaneously contracting lymphatic vessels. Filled dots represent data measured in NaCl-290 (n = 11) or NaCl-324 (n = 8) solutions respectively; hollow dots represent data measured in Cl-290 (n = 10) or Na-324 (n = 13) conditions respectively; gray diamonds represent data measured in M-control conditions (n = 42). (D) Effect of changes in osmolarity through solutions with variable NaCl on lymph flow (J_lymph) diaphragmatic lymphatic vessels (n = 19) displaying intrinsic pumping mechanism. Short term effect (hollow dot) displayed a J_lymph sigmoidal fit (dashed line), whereas long term response (filled dots) tended to zero J_lymph (solid line). (E) Effect of changes in osmolarity through solutions with variable Na⁺ (Na-324 solution) or Cl⁻ (Cl-290 solution) content in the perfusing solution on J_lymph in diaphragmatic spontaneously contracting lymphatic (n = 23). Short term effect (hollow dot) displays a J_lymph sigmoidal fit (dashed line), whereas long term response (filled dots) tended to zero J_lymph (solid line).