. 2018 Jul 5;9:961. doi: 10.3389/fpls.2018.00961

Table 3.

Protein content and SRT, phosphoenolpyruvate carboxylase (PEPC) and cytochrome c oxidase (COX) activities in different fractions obtained in the course of WM purification.

Fraction	Protein (mg)	SRT			PEPC		COX

		TA^a	% Inhibition by NAM	(SA^b∗, E^∗)	TA^c	(SA^d, E)	TA^e	(SA^f, E)
Homogenate	1000	429000 ± 58000	90.2	(387, 1.00)	52000 ± 4750	(52, 1.00)	13800 ± 720	(13.8, 1.00)
Pellet	35.1 ± 3.8	3300 ± 360	100	(94, 0.24)	1544 ± 170	(44, 0.85)	1035 ± 82	(29.5, 2.14)
Mitochondria	4.1 ± 0.3	720 ± 85	100	(176, 0.45)	49 ± 6	(12, 0.23)	1566 ± 185	(382, 28)

^ang of hSIRT1 eq.; ^bng hSIRT1 eq. ⋅ mg^-1 protein; ^cnmol of NADH oxidized ⋅ min^-1; ^dnmol NADH oxidized ⋅ min^-1. mg^-1 protein; ^enmol of oxygen taken up ⋅ min^-1; ^f nmol of oxygen taken up ⋅ min^-1 ⋅ mg^-1 protein; ^∗values are obtained by using the NAM-sensitive activity. Protein content and enzymatic activities were carried out as reported in Section “Materials and Methods”. For each enzyme, total activity (TA) and specific activity (SA) with respect to 1 g homogenate proteins, as well as the enrichment (E) of SA with respect to homogenate, are reported for all tested fractions. For SRT activity, % of inhibition by 100 mM is also reported. Data are expressed as mean value ± S.D. (n = 3 different experiments).