Figure 7.

Germline activity is required for HSN burst firing and the active state. A, Representative HSN Ca²⁺ traces in untreated (top) and FUDR-treated (bottom) adult animals. B, Representative HSN Ca² traces in adult glp-1(or178ts) sterilized animals (L1s shifted to 25°C for 24 h and raised to adults at 15°C) (top) and glp-1(or178ts) fertile animals (raised at 15°C) (bottom). Arrowheads indicate egg laying events. C, Cumulative distribution plots of instantaneous HSN Ca²⁺ transient peak frequencies (and intertransient intervals) of adult HSN Ca²⁺ activity. *p < 0.0001, Kruskal–Wallis test. D, Scatterplots showing total time spent by each individual with HSN transients ≤30 s apart in untreated (green filled circles) and FUDR-treated (green open circles) wild-type animals or fertile (blue filled circles) or sterile (blue open circles) glp-1(or178ts) mutant animals. *p ≤ 0.0001, one-way ANOVA. Error bars indicate 95% confidence intervals for the mean.