Figure 4.

α2δ-1Tat peptide abrogates the potentiating effect of Ang II on presynaptic and postsynaptic NMDAR activity in spinally projecting PVN neurons. A, B, Original recording traces and mean changes of NMDAR currents elicited by the puff application of 100 μmol/L NMDA to labeled PVN neurons in brain slices treated with 1 μmol/L control peptide plus 2 μmol/L Ang II (n = 13 neurons) or 1 μmol/L α2δ-1Tat peptide plus 2 μmol/L Ang II (n = 9 neurons). C, D, Representative traces and cumulative plots show mEPSCs of labeled PVN neurons before bath application of 50 μmol/L AP5 (Baseline), with bath application of 50 μmol/L AP5 (AP5), and after washout (Washout) in brain slices treated with control peptide plus Ang II or α2δ-1Tat peptide plus Ang II. E, F, Mean changes show the frequency and amplitude of mEPSCs in labeled PVN neurons treated with control peptide plus Ang II (n = 9 neurons) or with α2δ-1Tat peptide plus Ang II (n = 8 neurons). Data are presented as means ± SEM. *p < 0.05, **p < 0.01 compared with the baseline of the control peptide plus Ang II group.