Fig. 8.

The influence of absence of SAM or UDPGA on the glucuronidation and methylation of luteolin by RLS9 in UGT–COMT coreactions. Three different concentrations (1, 5, and 10 μM) of luteolin were used in the experiment. (A1–A3) 1, 5, and 10 μM luteolin was incubated in the absence and presence of SAM, respectively; (B1–B3) 1, 5, and 10 μM luteolin was incubated in the absence and presence of UDPGA, respectively. The RLS9 concentration was 0.25 mg/mL, and the incubation time was 30 minutes. All incubations were performed in triplicate. Each column represents the mean of three determinations. The error bar represents the S.D. of the mean (n = 3, mean ± S.D.). Significant differences (P < 0.05, marked by *) compared with the corresponding control group were described.