. 2017 Mar;45(3):306–315. doi: 10.1124/dmd.116.073619

TABLE 2.

Apparent kinetic parameters of luteolin and Lut-7-G methylation obtained from RLS9

Calculations were based on curve fitting using the Michaelis–Menten equation or biphasic model, as described in Materials and Methods. Data are expressed as the mean ± S.D., n = 3.

	Metabolites	K_m (μmol/L)	V_max (nmol/mg/min)	CL_int (mL/min/mg)
Luteolin	Chrysoeriol^a	33.8 ± 4.1	1.06 ± 0.05	0.03 ± 0.01
Luteolin	Diosmetin^a	17.9 ± 1.7	0.92 ± 0.14	0.05 ± 0.01
Lut-7-G	Chr-7-G^b	8.9 ± 0.6	0.38 ± 0.01	0.04 ± 0.01
Lut-7-G	Dio-7-G^b	15.1 ± 1.1	0.67 ± 0.02	0.04 ± 0.01

^{^a}

Biphasic equation.

^{^b}

Michaelis–Menten equation.