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. 2017 Dec 26;2(3):nzy002. doi: 10.1093/cdn/nzy002

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© 2018, Stamm et al.

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits noncommercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Trend of decreased HDAC6 protein in the ventral prostate lobe with broccoli sprouts. (A, B) HDAC6 was detected using immunohistochemistry in the prostate lobes of TRAMP mice fed control, or 15% broccoli sprout diet. Chromagen Nova Red; counterstain hematoxylin. (A) Representative images of HDAC6 staining in ventral prostate lobe at 28 wk of age taken at 400× magnification. (B) HDAC6 staining intensity quantification in which staining is expressed as a percentage of all possible color. Significant differences between samples were calculated using 2-factor ANOVAs with results detailed in individual tables for each prostate lobe (n = 6–16). Broc., broccoli; HDAC6, histone deacetylase 6; TRAMP, transgenic adenocarcinoma of the mouse prostate.