FIGURE 5.

Broccoli sprouts decreased H3K18ac in prostate epithelial cells. (A, B) H3K18ac was detected using immunohistochemistry in the prostate lobes of TRAMP mice fed control or 15% broccoli sprout diet. Chromagen Nova Red; counterstain hematoxylin. (A) Representative images of H3K18ac staining in indicated prostate lobes at 28 wk of age taken at 400× magnification. (B) H3K18 acetylation was quantified on a scale from 0 to 3. Significant differences between samples were calculated using 2-factor ANOVAs with results detailed in individual tables for each prostate lobe (n = 9–18). Bonferroni post-tests were used to determine differences between mice fed a control and broccoli sprout diet where *** indicates a significant difference between the groups at P < 0.001. Broc., broccoli; H3K18ac, acetyl-histone H3 lysine 18; TRAMP, transgenic adenocarcinoma of the mouse prostate.