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QUANTITATIVE RT’PCR CONFIRMS THE UPREGULATION OF A2M IN hESC-DERIVED ASTROCYTES
| ΔCt (hESC-Astrocyte) | ΔΔCt | Upregulation in Astrocytes | |
|---|---|---|---|
| B2M (reference gene) | −2.8 ± 0.05 | — | — |
| A2M | 2.6 ± 0.03 | 5.4 | 42-fold |
The fold change (estimated with the ΔΔCt method) is very close to the microarray measurement.
