Fig. 5.

Higher desiccation stress in Bay-0 corresponds to earlier stomatal opening during recovery. (A) Relative water content in intermediary leaves before submergence (pre-sub), after 5 d of submergence (0h), and subsequent recovery time points (n = 15). (B) Hourly water loss of 10-leaf-stage rosettes after detachment from roots immediately upon desubmergence (0 h) compared with the initial fresh weight (n = 30). (C) Stomatal width aperture (based on width/length ratio) measured using stomatal imprints on the adaxial side of intermediary leaves (n = 85 to 227) of plants before treatment (pre-sub), and subsequent recovery time points. (D) Stomatal aperture of epidermal peels from intermediary leaves of plants grown under control conditions and incubated in 0, 50, or 100 µM ABA (n = 180). (E) ABA quantification in intermediary leaves of Bay-0 and Lp2-6 recovering from 5 d of submergence and corresponding controls (n = 3). Data represent mean ± SEM. Different letters represent significant difference, and asterisks represent significant differences between the accessions at the specified time point (P < 0.05; B, two-way ANOVA with Tukey’s multiple comparisons test; E, one-way ANOVA with planned comparisons on log-transformed data).