Fig. 4.
Overproduction of the cGAMP synthase DncV results in degradation of phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) and release of free fatty acids (FFAs) in a CapV-dependent fashion. TLC plates of (A) polar lipids and (B) neutral lipids extracted at 0, 30, and 60 min postinduction with 1 mM IPTG from El Tor V. cholerae WT carrying pDncV or pDncVmut and from the El Tor V. cholerae ΔcapV mutant carrying pDncV. Plates are representative of three independent experiments. The PE, PG, and FFA standards are dioleoyl-PE, dioleoyl-PG, and linoleic acid, respectively. (C) Quantification (molar percentage of total lipids) by GC of PG, PE, and free fatty acids (FFAs) from A and B. Graphs show the mean of three independent experiments.