Fig. 6.

Overproduction of the cGAMP synthase DncV is detrimental to membrane integrity. Images depict the cell morphology (Top, bright field) and membrane integrity (Bottom, FM 4-64) of El Tor V. cholerae WT carrying pDncV 2 h postinduction with (Right) or without (Left) 100 µM IPTG. Expression of dncV leads to a loss of cellular integrity (Top Right) and altered fluorescent labeling of the cell membrane (Bottom Right). Arrows indicate affected ghost cells. Images are representative of six independent experiments.