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Primer-template duplex stabilized by primase. (A) Immobilized elongation assay with wild-type primase or PriSLXR72A. Beads, the product associated with the immobilized template; SN, the product in the supernatant. Lanes 13 and 14 show the bead-associated product after SDS treatment. (B) Quantified product distribution. For each reaction, total product intensity was normalized to 1. *The 9-nt product position for each primer is indicated.
