Fig. 7.

Seeding experiments. (A) Normalized ThT fluorescence as a function of time of mutant A21G, E22Q, E22G, E22K, and D23N. Seeds of the same peptide were added at time 0 at concentrations of 0.04%, 0.2%, 1%, 5%, 10%, and 30% monomer equivalents, and the monomer concentration is indicated in each panel. All samples contain 6 $\mathrm{\mu }$M of ThT, 20 mM sodium phosphate, and 200 $\mathrm{\mu }$M of EDTA at pH 8.0. Data at additional monomer concentrations are shown in SI Appendix, Fig. S6. (B) Aggregation half time as a function of the logarithm of the seed concentration for A21G, E22Q, E22G, E22K, and D23N, at one or three different monomer concentrations as indicated in each panel.