Fig. 8.

Effect of varying ionic strength on the half times of aggregation for the different mutants. Half times are plotted against initial monomer concentration on a double logarithmic plot. Bottom Right compares the combined rate constant of secondary nucleation and elongation for the different mutants and ionic strengths. Lower, standard, and higher ionic strengths are 24 mM, 60 mM, and 162 mM, respectively. While D23N is not significantly affected by changes in ionic strength, both E22K and E22Q are sped up significantly at higher ionic strengths, suggesting that charge still plays a role during the aggregation of these mutants.