Fig. 3.

Characterization of the differentiation status of AOs. (A) Fold changes in expression levels of cell-type markers (Left) and serine proteases (Right) in the organoids cultured in PD medium versus those cultured in AO medium at the indicated days. Data show the mean and SD of two lines of organoids. *P < 0.05; **P < 0.01. (B) The percentages of individual cell types in the organoids cultured in PD medium and AO medium. The representative histograms of one organoid line are shown. (C) Fold change in positive cell percentages in organoids cultured in PD medium versus those in AO medium. Data are presented as the mean and SD of four independent experiments. *P < 0.05.