Fig. 3.

The formation of polymeric SUMO2 chains is not essential for the regulation of IFN by sumoylation. THP-1 monocytes were transduced with lentiviral constructs encoding GFP, wild-type SUMO2 (WT), an unconjugatable diglycine mutant (GG-AA), a K11R mutant, or a SUMO2 mutant with all known conjugatable lysines mutated to arginines (5KR). Each cell line was then transduced with the indicated lentiCRISPR constructs. Evaluation of IFNB1, IFI27, and ISG15 mRNA expression by quantitative RT-PCR is shown in PMA-differentiated THP-1 cells. Statistical analysis was performed using a two-way ANOVA and comparing WT, GG-AA, K11R, and 5KR transduced cells to GFP-transduced cells, correcting for multiple comparisons using the Holm–Sidak method. n = 3 where n is the number of unique polyclonal cell lines. n.s., not significant; *P ≤ 0.05; ***P ≤ 0.001.