Fig. 6.

Clinical impact of PLEKHA7 in EOC patients. a Western blotting on total cell lysates from MCAs present in ascites from HG-SOC patients (n = 13). Caco2 lysates were used as positive control of PLEKHA7 expression. Claudin-4 was included as epithelial marker. Immunoblottings were performed with Abs against the proteins reported on the left. β-actin was used as control of gel loading. b Representative IF performed on a fixed sample of HG-SOC MCAs (sample #11) with anti-E-cadherin (cadh) and anti-PLEKHA7 Abs. c Representative images of the IHC performed with anti-PLEKHA7 Ab on HG-SOCs. The reaction on the fallopian tube epithelium (FT) was considered as a positive control. The empty black box in the left panel highlights the image reported in the right panel at higher magnification. d Meta-analysis, as described in Methods section, for evaluation of PLEKHA7 expression intensity in OSE and EOCs of different histotypes. Asterisks indicate statistically significant values by Student’s t test (p ˂ 0.001). e Kaplan-Meyer curves reporting the PFS and OS analyses on patient selected for PLEKHA7 expression