Fig. 1.

JQ1 inhibits growth and induces apoptosis in AML cell lines. a AML cell lines were treated with JQ1 at gradient concentration for 48 h and the viabilities were measured using CellTiter-Glo. The growth rates were normalized to cells without treatment. b Change of c-Myc protein level after JQ1 treatment for 48 h. c AML cell lines were treated with JQ1 at 300 nM or 600 nM for 48 h before they were stained with PI and Annexin V and analyzed using flow cytometry. Percentages of apoptotic cells at early and late stage (i.e. Annexin V positive) were shown. d C-PARP protein level significantly increased after JQ1 treatment for 24 h. e Activation of intrinsic apoptosis pathway. OCI-AML-2 and OCI-AML-3 Cells were treated with JQ1 at respective IC₅₀ and harvested at different time points before immunoblotting. f Caspase activity assay of dose-dependent JQ1-treated AML cell lines. For each concentration, triplicate samples were prepared. The relative activities were normalized to culture medium