Fig. 2.

TXNIP partially mediates the JQ1-induced apoptosis through activating the p38 MAPK pathway instead of increasing ROS. TXNIP (a) mRNA and (b) protein level after JQ1 treatment. Cells were treated with JQ1 at respective IC₅₀ for 24 h before being harvested and lysed. For real time qPCR results, means and standard errors were shown. c OCI-AML-3 cells were transfected with either pLKO.1 or pLKO.1-shTXNIP vectors and treated with JQ1 or DMSO for 24 h. d Relative quantification of apoptotic cells percentage using flow cytometry. Triplicates were performed and standard errors were shown. Percentages of apoptotic cells were normalized to DMSO-treated control. e Flow cytometry results showing the ROS level change in OCI-AML2 and MOLM-14 cells. Cells were treated with JQ1 at respective IC₅₀ and harvested at different time points. f Percentages of apoptotic cells measured by flow cytometry. Cells were pre-treated with NAC for 1 h before adding JQ1. g AML cells were treated with JQ1 for 24 h and subjected to immunoblot analysis with primary antibodies indicated. h p38 MAPK inhibitor rescued cells from JQ1-induced apoptosis. Percentages of apoptotic cells were shown (*: p < 0.05, **: p < 0.01)