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. Author manuscript; available in PMC: 2018 Dec 27.
Published in final edited form as: Nature. 2018 Jun 27;559(7712):109–113. doi: 10.1038/s41586-018-0257-1

Figure 3. The crypt response to H. polygyrus is a generalized response to tissue injury.

Figure 3

a–d, Mice were treated with 20 µg anti-TCRβ and analyzed 24 hours later for Lgr5-GFP and Sca-1 in crypt cells (a–b) or Sca-1 and EdU (c–d). n=6/group (a–b). n=4/group; scale bars, 100 µm (c–d). e–j, Mice were treated with 10 gray irradiation and analyzed at 3 days for Lgr5-GFP and Sca-1 in crypt cells (e–h) or Lgr5-GFP and Ki67 (i–j). n=4 (Lgr5, untreated), 5 (Lgr5, irradiated), or 6 (Sca-1) (e–f). n=3/group; scale bars, 100 µm (g–j). k–m, Lgr5DTRGFP/+ mice were treated with diphtheria toxin (DT) and analyzed for Lgr5-GFP and Sca-1 in crypt cells at 24 hours (k–l) or indicated time points (m). n=3 (Lgr5, untreated), or 4 (all others) (k–l). n=3 (day 0) or 4 (days 1, 2, 4) (m). Unpaired, two-tailed Mann-Whitney test (b, f, l) or unpaired, two-tailed t tests (m); mean ± S.D. (b, f, l, m). * P < 0.05, ** P < 0.01, **** P < 0.0001.